Composite

Part:BBa_K4119010:Design

Designed by: Chengtai Yin   Group: iGEM22_NJTech_China   (2022-09-30)


Plac->ΔperR->self-target->Cpa fdx terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 368
    Illegal PstI site found at 1597
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 368
    Illegal PstI site found at 1597
    Illegal NotI site found at 3487
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 368
    Illegal BglII site found at 428
    Illegal XhoI site found at 3359
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 368
    Illegal PstI site found at 1597
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 368
    Illegal PstI site found at 1597
    Illegal NgoMIV site found at 3791
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

none

References